SeqTec: NGS Bioinformatics Toolkit
Runnable code examples for every bioinformatics tool covered in The Complete Guide to Next-Generation Sequencing.
Note
This project was developed by Kevin Korfmann as an educational resource for next-generation sequencing and bioinformatics. Drafting, code examples, and explanations were produced with substantial assistance from Claude, an AI assistant by Anthropic. All content has been reviewed and curated by the author, but readers should verify implementations against primary literature.
Getting Started
Data Formats
Tools
- Tools
- Quality Control
- Alignment
- SAM/BAM Processing
- Interval Operations
- Genome Assembly
- Assembly Quality Control
- Genome Annotation
- Variant Calling
- Variant Annotation
- Variant Processing
- Quantification
- Differential Expression
- Single-Cell Analysis
- Metagenomics
- Epigenomics
- Basecalling
- Data Access
- Visualization
Workflows
Infrastructure
Reference